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Here, we solved the crystal structure of the complex of the inhibitor with archetypal astacin from crayfish, which is a useful model of human ovastacin. This inhibition is exerted by blocking active-site cleft sub-sites upstream and downstream of Lady massage in Furth catalytic zinc ion, but not those flanking the scissile bond.
However, contrary to the mouse complex, which Dreiejch obtained with fetuin-B nicked at a single site but otherwise intact, most of the CTR was Dreieoch removed during crystallization of the human complex. Moreover, the two complexes present in the crystallographic asymmetric unit Free mobile Dormagen in the relative arrangement of CY1 and CY2, while the two complexes found for the mouse complex crystal structure were equivalent.]